How do you know you successfully transformed the cells with pGlo

This lab report will cover the transformation of E.coli  and the restriction digest of the plasmid (labs 5 to 8).

Use the Standard lab report format and include the following

– Overall objective

– Background to what you were trying to do

– Methods – used to create reagents and used for the transformation, plasmid isolation, restriction digest and agarose gels.  Create sub section for each

Transformation

– What was the selectable marker?

– How do you know you successfully transformed the cells with pGlo

– What was the most critical step in the transformation process

Plasmid preparation

– Why does only the E. coli grown in ara/amp fluoresce

Restriction digest

– What size fragments would you expect when cutting the plasmid with the two enzymes used?

 

– Are the results of the plasmid what you expected.

– Are the two fragments the sizes you expected ?